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KMID : 1007520180270061761
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Food Science and Biotechnology
2018 Volume.27 No. 6 p.1761 ~ p.1769
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Antioxidant and anti-inflammatory functionality of ten Sri Lankan seaweed extracts obtained by carbohydrase assisted extraction
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Fernando I. P. Shanura
Sanjeewa K. K. Asanka
Samarakoon Kalpa W.
Lee Won-Woo
Kim Hyun-Soo
Ranasinghe P.
Gunasekara U. K. D. S. S.
Jeon You-Jin
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Abstract
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Enzyme-assisted extraction is a cost-effective, safe, and efficient method to obtain bioactives from plant materials. During this study, 10 different marine algae from Sri Lanka were individually extracted by using five commercial food-grade carbohydrases. The enzymatic and water extracts of the seaweeds were analyzed for their antioxidant and anti-inflammatory activities. The highest DPPH, hydrogen peroxide (H2O2) and intracellular H2O2 scavenging abilities were observed from the Celluclast extract of Sargassum polycystum (CSp). CSp exerted protective effects against oxidative stress-induced cell death in hydrogen peroxide-induced Chang cells and in model zebrafish. The Celluclast extract of Chnoospora minima (CCm) showed the strongest anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages (IC50?=?44.47 μg/mL) and in model zebrafish. CCm inhibited the levels of iNOS, COX-2, PGE2, and TNF-α in LPS stimulated RAW 264.7 macrophages. Hence, CSp and CCm could be utilized in developing functional ingredients for foods, and cosmeceuticals.
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KEYWORD
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Enzyme-assistant extraction, Celluclast, Marine algae, Sargassum polycystum, Chnoospora minima, Antioxidant
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